Scientific Publications


Development of the Thermophilic Filamentous Fungus Thermothelomyces heterothallica C1 into a next-generation production platform for human and animal vaccines and drugs  September 2022

Toxicity and Local Tolerance of a Novel Spike Protein RBD Vaccine Against SARS-CoV-2, Produced Using the C1 Thermothelomyces Heterothallica Protein Expression Platform May 5, 2022, Click here for the link to the paper in Toxicologic Pathology

Vaccines – The Highly Productive Thermothelomyces heterothallica C1 Expression System as a Host for Rapid Development of Influenza Vaccines January 20, 2022 Click here for the link to the paper in Analytical and Bioanalytical Chemistry

Science Direct, January 19, 2022 A recombinant SARS-CoV-2 receptor-binding domain expressed in an engineered fungal strain of Thermothelomyces heterothallica induces a functional immune response in mice
Click here for the link to the full article


In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI-MS spectrum November 5, 2021 Click here for the link to the paper in Analytical and Bioanalytical Chemistry

Andrea Aebischer, Kerstin Wernike, Patricia Konig, Kati Franzke, Paul J. Wichgers Schreur, Jeroen Kortekaas, Marika Vitikainen, Marilyn Wiebe, Markku Saloheimo, Ronen Tchelet, Jean-Christophe Audonnet, Martin Beer: Development of a Modular Vaccine Platform for Multimeric Antigen Display Using an Orthobunyavirus Model.  Vaccines 2021: Click here for the link to the paper in Vaccines MDPI



van Gool, M.P., van Muiswinkel, G.C.J., Hinz, S.W.A., Schols, H.A., Sinitsyn, A.P., Gruppen, H. “Two novel GH11 endo-zylanases from Myceliophthora thermophila C1 act differently toward soluble and insoluble xylans”.Enzyme and Microbial Technology 53 (2013) 25-32.

Koutaniemi, S., van Gool, M.P., Juvonen, M., Jokela, J., Hinz, S.W., Schols, H.A., Tenkanen, M. “Distinct roles of carbohydrate esterase family CE16 acetyl esterases and polymer-acting acetyl xylan esterases in xylan deacetylation.”Journal of Biotechnology 168 (2013) 684-692.


van Gool, M.P., van Muiswinkel, G.C.J., Hinz, S.W.A., Schols, H.A.,  Sinitsyn, A.P.,  Gruppen, H. “Two GH10 endo-xylanases of Myceliophthora thermophila C1 with and without cellulose binding module act differently towards soluble and insoluble substrates”. Bioresource Technology 2012: doi: 10.1016/j.biotech.2012.05.117.

Klyosov, A.A., Dotsenko, G.S., Hinz, S.W.A., and Sinitsyn, A.P. “Structural features of β-(1>4)-D-galactomannans of plant origin as a probe for β-(1>4)- mannanase polymeric substrate specificity.”Carbohydrate Research May 2012: 352:65-69.

Dotsenko, G.S., Sinitsyn, O.A., Hinz, S.W.A., Wery, J. and Sinitsyn, A.P. “Characterization of a GH family 3 b-glycoside hydrolase from Chrysosporium lucknowense and its application to the hydrolysis of b-glucan and xylan.”Bioresource Technology May 2012: 112:345–349.

Kühnel, S., Pouvreau, L., Appeldoorn, M.M., Hinz, S.W.A, Schols, H.A. and Gruppen, H. “The Ferulic Acid Esterases of Chrysosporium Lucknowense C1: Purification, Characterization and Their Potential Application in Biorefinery.” Enzyme and Microbial Technology January 2012: 5;50(1):77-85.


Berka, Randy et al. “Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris.” Nature Biotechnology October 2011.

Punt, Peter J., Levasseur, Anthony, Visser, Hans, Wery, Jan and Record, Eric. “Fungal Protein Production: Design and Production of Chimeric Proteins.” The Annual Review of Microbiology October 2011: 65: 57–56.

Visser, Hans, Joosten, Vivi, Punt, Peter J., Gusakov, Alexander V., Olson, Phil T., Joosten, Rob, Bartels, Jeffrey, Visser, Jaap, Sinitsyn, Arkady P., Emalfarb, Mark A., Verdoes, Jan C., and Wery, Jan.  “Development of a mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila isolate, previously known as Chrysosporium lucknowense C1.” Industrial Biotechnology June 2011.

Pouvreau, L., Jonathan, M.C., Kabel, M.A., Hinz, S.W., Gruppen, H. and Schols, H.A. “Characterization and mode of action of two acetyl xylan esterases from Chrysosporium lucknowense C1 active towards acetylated xylans.” Enzyme and Microbial Technology August 2011: 49(3):312-320.

Pouvreau, Laurice, Joosten, Rob, Hinz, Sandra W.A., Gruppen, Harry and Schols, Henk A. “Chrysosporium lucknowense C1 arabinofuranosidases are selective in releasing arabinose from either single or double substituted xylose residues in arabinoxylans.” Enzyme and Microbial Technology April 2011: 7; 48(4-5):397-403.

Kühnel, S., Westphal, Y., Hinz, S.W.A, Schols, H.A. and Gruppen, H. “Mode of action of Chrysosporium lucknowense C1 a-L-arabinohydrolases.” Bioresource Technology January 2011: 102(2):1636-1643.

Gusakov, A.V.  “Alternatives to Trichoderma reesei in biofuel production.”  Trends in Biotechnology, 2011, v.29, No. 9, p.419-425


Kühnel, S., Hinz, S.W.A., Pouvreau, L., Wery, J., Schols, H.A. and Gruppen, H. “Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.” Bioresource Technology November 2010: 101(21):8300-8307.

Westphal, Y., Kühnel, S., de Waard, P., Hinz, S.W.A., Schols, H.A., Voragen, A.G. and Gruppen, H. “Branched arabino-oligosaccharides isolated from sugar beet arabinan.” June 2010: 345(9):1180-1189.

Kühnel, S., Hinz, S.W.A., Pouvreau, L., Wery, J., Schols, H.A. and Gruppen, H. “Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.” Bioresource Technology November 2010: 101(21):8300-8307.


Gusakov, A.V., Salanovich, T.N., Antonov A.I., Ustinov, B.B, Okunev, O.N., Burlingame, R., Emalfarb, M., Baez, M. and Sinitsyn, A.P. “Design of highly efficient cellulase mixtures for enzymatic hydolysis of cellulose.” Biotechnol Bioengineering August 2007: 97(5):1028-1038.

Verdoes, J.C., Punt, P.J., Burlingame, R.P., Bartels, J., van Dijk, R., Slump, E., Meens, M., Joosten, R. and Emalfarb, M. “A dedicated vector for efficient library construction and high throughput screening in the hyphal fungus Chrysosporium lucknowense.” Industrial Biotechnology Spring 2007: 48-57.


Burlingame, R.P. and Chandra R. “Gene discovery and protein production technology: An integrated system to discover, develop and manufacture enzymes and other proteins.” Industrial Biotechnology 2005: 1(1):35-37.

Gusakov, A.V., Sinitsyn, A.P., Salanovich, T.N., Bukhtojarov, F.E., Markov, A.V., Ustinov, B.B., van Zeijl, C., Punt, P., Burlingame, R.  “Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense.”  Enzyme and Microbial Technology, 2005, v.36, No. 1, p.57-69


Gusakov, A.V., Sinitsyn, A.P., Markov, A.V., Sinitsyna, O.A., Ankudimova, N.V.,Berlin, A.G.  “Study of protein adsorption on indigo particles confirms the existence of enzyme-indigo interaction sites in cellulase molecules.” Journal of Biotechnology, 2001, v.87, No. 1, p.83-90

Sinitsyn, A.P., Gusakov, A.V., Grishutin, S.G., Sinitsyna, O.A., Ankudimova, N.V.  “Application of microassays for investigation of cellulase abrasive activity and backstaining.”  Journal of Biotechnology, 2001, v.89, No. 2, p.233-238